MAB3199

Anti-E-Cadherin Antibody, clone 67A4

clone 67A4, Chemicon^®, from mouse

• Synonym(s): CD324
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 67A4, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_004360.2
• UniProt accession no.: P12830
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CDH1(999)

Description

Specificity

Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E-cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.

Antigen is expressed in non-neural epithelial tissues.

Immunogen

T-47D breast carcinoma line

Application

Anti-E-Cadherin Antibody, clone 67A4 detects level of E-Cadherin & has been published & validated for use in FC & WB.

Immunoblotting: 123kD in membrane fractions; MCF-7 membranes positive control. Confluent cells were washed three times with ice-cold PBS and lysed in 50mM HEPES pH 7.5, 150mM NaCl, 1mM EDTA, 10% glycerine, 1% triton X-100, 20mM Na(4)P(2)O(7), 2mM Na-vanadate, 100mM NaF, 2mM PMSF, 20μg/mL leupeptin, 1mM pNPP(para-nitrophenylphosphate), 20μg/mL aprotinin, and 200μM NH(4)-molybdate. Crude extracts were clarified by centrifugation (10,000 x g 10 min), and supernatants were taken for immunoprecipitation. Protein concentration of cleared lysates were determined by BCA protein assay. {Buhring, et al Luekemia 10:106-116, 1996}.



FACS Analysis

Functional blocking of E-cadherin

Does not work well for immunoprecipitation

Original paper, Buhring, H et al Leukemia 10:106-116, 1996.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Linkage

Replaces: 04-1103

Physical form

Format: Purified

Protein A purified immunoglobulin. Liquid in 0.02M PBS with NaCl, pH=7.6, 0.1% Sodium azide.

Storage and Stability

Maintain 2-8°C in undiluted aliquots for up to 12 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable