ABS1006
Anti-PCSK9 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
NARC-1, PC9, Subtilisin/kexin-like protease PC9, Proprotein convertase 9, Neural apoptosis-regulated convertase 1, Proprotein convertase subtilisin/kexin type 9
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PCSK9(255738)
General description
PCSK9 protein is an important protein in the regulation of plasma cholesterol homeostasis. PCSK9 binds various lipid receptor protein members including low density lipoprotein receptors (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2) and promotes their degradation or sequestration. In neurons, PCSK9 regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. PCSK9 is localized in cytoplasm but it is also secreted and thus can be found in endosomes, lysosomes, and endoplasmic reticulum and Golgi as well. PCSK9 is expressed in normal cells such as Schwann cells and pancreatic epithelial cells but PCSK9 is also expressed in many cancers and cancer cells lines as well.
Immunogen
His-tagged recombinant protein corresponding to Human PCSK9.
Application
Research Category
Signaling
Metabolism
Signaling
Metabolism
Research Sub Category
Lipid Signaling
Liver Physiology
Lipid Signaling
Liver Physiology
This Anti-PCSK9 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of PCSK9.
Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in treated HepG2 cell lysate (Jeong, H. J., et al. J. Lipid Res. 2008. 49: 399–409.)
Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in siRNA transfected HepG2 cells without and with BBR treatment (Data compliments of Sahng Wook Park of Yongsei University College of Medicine).
Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in siRNA transfected HepG2 cells without and with BBR treatment (Data compliments of Sahng Wook Park of Yongsei University College of Medicine).
Quality
Evaluated by Immunocytochemistry on HepG2 cells
Immunocytochemistry Analysis: 8.0 µg/mL of this antibody detected PCSK9 in HepG2 cells.
Immunocytochemistry Analysis: 8.0 µg/mL of this antibody detected PCSK9 in HepG2 cells.
Target description
~75 kDa observed
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 30% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Sterol-dependent regulation of proprotein convertase subtilisin/kexin type 9 expression by sterol-regulatory element binding protein-2.
Jeong, HJ; Lee, HS; Kim, KS; Kim, YK; Yoon, D; Park, SW
Journal of Lipid Research null
Hepatocyte nuclear factor 1alpha plays a critical role in PCSK9 gene transcription and regulation by the natural hypocholesterolemic compound berberine.
Li, H; Dong, B; Park, SW; Lee, HS; Chen, W; Liu, J
The Journal of Biological Chemistry null
Strong induction of PCSK9 gene expression through HNF1alpha and SREBP2: mechanism for the resistance to LDL-cholesterol lowering effect of statins in dyslipidemic hamsters.
Dong, B; Wu, M; Li, H; Kraemer, FB; Adeli, K; Seidah, NG; Park, SW; Liu, J
Journal of Lipid Research null
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