ABN55
Anti-Netrin-1 Antibody
serum, from rabbit
Synonym(s):
netrin 1, mouse, homolog of, Netrin 1, Ntn1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Ntn1(18208)
General description
Netrin-1 is a secreted, bifunctional ligand belonging to the Netrin family; a group of proteins known to act as versatile, chemotropic axon guidance cues for CNS commissural and peripheral motor axons. Netrin-1 is necessary for spinal cord oligodendrocyte development. There are two receptors with which Netrin-1 is associated and that will cause it to be either a chemoattractant or a chemorepellant for axons. Receptor Deleted in Colorectal Cancer (DCC) regulates the attraction of axons to Netrin-1, and UNC5 mediates axon repulsion from Netrin-1. Studies have shown Netrin-1 to function as a survival factor through its relationship to its receptors, inhibiting the onset of apoptosis. Netrin-1 has also been implicated in colorectal tumorigenesis via its apoptotic regulatory role. Expression of Netrin-1 has been detected in adult axons and oligodendrocytes, and is also widely observed in developing embryonic mesodermal tissues and nervous system.
Specificity
Other homologies: Human (94% sequence homology).
Immunogen
Epitope: Unknown
Linear peptide corresponding to mouse Netrin-1.
Application
Anti-Netrin-1 Antibody detects level of Netrin-1 & has been published & validated for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Growth Cones & Axon Guidance
Quality
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1:10,000 dilution of this antibody detected Netrin-1 on 10 µg of mouse brain tissue lysate.
Western Blot Analysis: 1:10,000 dilution of this antibody detected Netrin-1 on 10 µg of mouse brain tissue lysate.
Target description
~68 kDa observed. Characteristic proteolytic fragments of Netrin-1 found in the CNS run at lower molecular weights.
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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