1.50461

SeQuant^® ZIC^®-pHILIC (5 µm) HPLC Columns

L x I.D. 150 mm x 4.6 mm

• Synonym(s): Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: SeQuant^® ZIC^®-pHILIC 5µm polymer 150 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC
• material: PEEK-lined stainless steel column
• Quality Level: 100
• Agency: suitable for USP L122
• product line: SeQuant^®
• feature: endcapped: no
• parameter: 200 bar pressure; 50 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 15 cm × 4.6 mm
• matrix: polymeric particle platform
• matrix active group: Zwitterion bonding phase, ZIC-pHILIC
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH: 2-10
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Description

General description

SeQuant^® ZIC^®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion, functional group as the silica based ZIC^®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.SeQuant^® ZIC^®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds. Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant^® ZIC^®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD). SeQuant^® ZIC^®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant^® ZIC^®-HILIC columns with identical functional groups, and SeQuant^® ZIC^®-cHILIC columns with complementary selectivity.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 40000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids