1.02449

Readybag^® Half FRASER broth with supplements

pkg of 60 pieces, according to FDA (BAM), ISO 11290, for Listeria spp.

• Synonym(s): Demi FRASER broth with supplements
• UNSPSC Code: 41106213

Properties

• Agency: APHA; ISO 11290
• Quality Level: 200
• reg. compliance: according to FDA (BAM)
• sterility: sterile; γ-irradiated
• form: medium granules (dehydrated (DCM))
• packaging: pkg of 60 pieces; pkg of (Each sachet contains 12.5 g of granulated Half Fraser broth to which 225 mL of sterile water is added)
• manufacturer/tradename: ReadyBag^®
• technique(s): microbiological culture: suitable
• pH: 7.0-7.4 (25 °C, 55 g/L in H₂O)
• solubility: 55 g/L
• application(s): food and beverages; microbiology; pathogen testing
• storage temp.: 15-25°C
• suitability: Listeria spp. (spp.)

Description

General description

Readybag^® Half FRASER broth with supplements come in pre-weighed and gamma-irradiated to speed up and simplify food pathogen testing routines. This helps to eliminate all the media preparation steps from the workflow. The Readybag^® single-use pouches remove the necessity of weighing, autoclaving, supplement handling, and extra lab space or equipment. It only requires the addition of sterile water before incubation.
The high nutrient content and the large buffer capacity of the Readybag^® Half FRASER broth (with supplements) create optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate forms a black complex of esculetin with iron (III) ions and facilitates the detection of β-D-glucosidase activity. But this reaction is not exclusive to Listeria spp. Hence, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media.

Application

Readybag^® Half FRASER broth (with supplements) is suitable for the primary selective enrichment of Listeria spp. from food and animal feed, environmental samples, and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable