07-049
Anti-Nedd4
Upstate®, from rabbit
Synonym(s):
E3 ubiquitin-protein ligase NEDD4, EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, Neural precursor cell expressed developmentally down-regulated protein 4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
species reactivity (predicted by homology)
human (based on 100% sequence homology)
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NDFIP1(80762)
General description
E3 ubiquitin-protein ligase NEDD4 (UniProt: P46934; also known as EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase Nedd4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4) is encoded by the Nedd4 (also known as KIAA0093, Nedd4-1) gene (Gene ID: 4734) in human. NEDD4 is a peripheral membrane protein that is recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, it follows IGF1R internalization and remains associated with early endosomes. Nedd4 can accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. It is shown to specifically ubiquitinate Lysine 63 in target proteins. It also monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. NEDD4 is reported to polyubiquitinate the epithelial sodium channel and any mutations that disrupt association between Nedd4 and sodium channel can lead to Liddle s syndrome, a hyperaldosteronism-like hypertension disease. NEDD4 can interact with viral proteins that contain a late-budding motif P-P-P-Y and this interaction is believed to be essential for viral particle budding of many retroviruses, like HTLV-1 Gag, and MLV Gag. It contains four WW domains (WW 1-4; aa 610-643; 767-800; 840-873; and 892-925) and one HECT domain (aa 984-1318). The WW domains mediate its interaction with PPxY motif-containing proteins.
Specificity
This rabbit polyclonal antibody specifically detects E3 ubiquitin-protein ligase Nedd4.
Immunogen
GST/His-tagged recombinant fragment corresponding to 68 amino acids from the N-terminal half of human Nedd4.
Application
Anti-Nedd4 Antibody, Cat. No. 07-049, is a rabbit polyclonal antibody that detects Nedd4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected NEDD44 in rat cerebral cortex, rat cerebellum, and human colon cancer tissue sections.
Quality
Evaluated by Western Blotting in rat kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nedd4 in rat kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nedd4 in rat kidney tissue lysate.
Target description
~110 kDa observed; 102.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 0.05% sodium azide.
Analysis Note
Control
Kidney cytoplasmic extracts.
Kidney cytoplasmic extracts.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Image analysis algorithms for immunohistochemical assessment of cell death events and fibrosis in tissue sections.
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O Staub et al.
The American journal of physiology, 272(6 Pt 1), C1871-C1880 (1997-06-01)
The epithelial Na+ channel (ENaC) was previously shown to be expressed in several Na(+)- and fluid-absorbing epithelia, particularly those of the kidney, colon, and lung. We have recently identified the ubiquitin-protein ligase Nedd4 as an interacting protein with ENaC and
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