374210
5-Bromoindoxyl acetate
98%
Synonym(s):
3-Acetoxy-5-bromoindole, O-Acetyl-5-bromoindoxyl
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About This Item
Empirical Formula (Hill Notation):
C10H8BrNO2
CAS Number:
Molecular Weight:
254.08
Beilstein:
168698
EC Number:
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
NACRES:
NA.22
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Quality Level
Assay
98%
form
powder
mp
130-132 °C (lit.)
solubility
ethanol: soluble 50 mg/mL, clear, colorless
functional group
bromo
ester
SMILES string
CC(=O)Oc1c[nH]c2ccc(Br)cc12
InChI
1S/C10H8BrNO2/c1-6(13)14-10-5-12-9-3-2-7(11)4-8(9)10/h2-5,12H,1H3
InChI key
KFTGECHXNQBTNZ-UHFFFAOYSA-N
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General description
5-Bromoindoxyl acetate is a halogenated heterocyclic compound. It is widely used as esterase substrate.
Application
5-Bromoindoxyl acetate may be used as substrate for the assay of insect esterases by employing DEAE (Diethylaminoethyl)-cellulose columns.
5-Bromoindoxyl acetate may be used:
- as esterase substrate to investigate the activity of non-specific esterase in the matrix of developing bovine enamel
- in histochemical studies of the nonspecific esterase of mouse epididymis
- as substrate to investigate the esterase activity in guinea-pig thyroid and mouse epididymis epithelial cells
- in staining procedure for the frozen sections of muscle fixed in buffered formaldehyde
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Complete staining of neuromuscular innervation with bromoindigo and silver.
McIsaac G and Kiernan JA.
Biotechnic & Histochemistry, 49(4), 211-214 (1974)
S Kirkeby et al.
Acta histochemica, 62(1), 44-56 (1978-01-01)
Esterase activity in guinea-pig thyroid and mouse epididymis epithelial cells has been studied using 5-bromoindoxyl acetate as substrate. The pattern of esterase activity in the thyroid of the guinea-pig is constant, irrespective of whether ferri-ferrocyanide (FFC) or certain copper compounds
S Kirkeby et al.
Archives of andrology, 6(2), 163-173 (1981-03-01)
Nonspecific esterase of mouse epididymis has previously been studied histochemically, using alpha naphthyl-acetate and 5-bromoindoxyl acetate techniques, as well as certain inhibitors. Epithelial cell types of the epididymis have been characterized, and certain esterase isozymes in a particular cell type
Afsharpour F and O'Brien RD.
Journal of Insect Physiology, 9(4), 521-529 (1963)
S Kirkeby et al.
Acta histochemica, 83(1), 11-19 (1988-01-01)
Electrophoretic separation of non-specific esterases and esterproteases from kidney, lung, and liver have been carried out in polyacrylamide gels. By use of zone electrophoresis, isoelectric focusing, and 2-dimensional electrophoresis it was found that most of the esterprotease bands had the
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