RPMI-1640 Media Formulation

RPMI-1640 Medium Dutch Modification
RPMI-1640 Medium HEPES Modification
RPMI-1640 Medium Modified
RPMI-1640 Medium Auto-Mod™ for Autoclaving

RPMI-1640 Medium Composition

Component	R0883 [1x] g/L	R1145 [10x] g/L	R1383 g/L	R1780 [1x] g/L	R2405 [1x] g/L	R6504 g/L	R8758 [1x] g/L
Inorganic Salts
Calcium Nitrate • 4H2O	0.1	1	0.1	0.1	0.1	0.1	0.1
Magnesium Sulfate (anhydrous)	0.04884	0.4884	0.04884	0.04884	0.04884	0.04884	0.04884
Potassium Chloride	0.4	4	0.4	0.4	0.4	0.4	0.4
Sodium Bicarbonate	2	—	—	2	2	—	2
Sodium Chloride	6	60	6	6	6	6	6
Sodium Phosphate Dibasic (anhydrous)	0.8	8	0.8	0.8	0.8	0.8	0.8
Amino Acids
L-Alanyl-L-Glutamine	—	—	—	—	0.4344	—	—
L-Arginine	0.2	2	0.2	—	0.2	0.2	0.2
L-Asparagine (anhydrous)	0.05	0.5	0.05	0.05	0.05	0.05	0.05
L-Aspartic Acid	0.02	0.2	0.02	0.02	0.02	0.02	0.02
L-Cystine • 2HCl	0.0652	0.652	0.0652	0.0652	0.0652	0.0652	0.0652
L-Glutamic Acid	0.02	0.2	0.02	0.02	0.02	0.02	0.02
L-Glutamine	—	—	0.3	0.3	—	0.3	0.3
Glycine	0.01	0.1	0.01	0.01	0.01	0.01	0.01
L-Histidine	0.015	0.15	0.015	0.015	0.015	0.015	0.015
Hydroxy-L-Proline	0.02	0.2	0.02	0.02	0.02	0.02	0.02
L-Isoleucine	0.05	0.5	0.05	0.05	0.05	0.05	0.05
L-Leucine	0.05	0.5	0.05	—	0.05	0.05	0.05
L-Lysine • HCl	0.04	0.4	0.04	—	0.04	0.04	0.04
L-Methionine	0.015	0.15	0.015	0.015	0.015	0.015	0.015
L-Phenylalanine	0.015	0.15	0.015	0.015	0.015	0.015	0.015
L-Proline	0.02	0.2	0.02	0.02	0.02	0.02	0.02
L-Serine	0.03	0.3	0.03	0.03	0.03	0.03	0.03
L-Threonine	0.02	0.2	0.02	0.02	0.02	0.02	0.02
L-Tryptophan	0.005	0.05	0.005	0.005	0.005	0.005	0.005
L-Tyrosine • 2Na • 2H2O	0.02883	0.2883	0.02883	0.02883	0.02883	0.02883	0.02883
L-Valine	0.02	0.2	0.02	0.02	0.02	0.02	0.02
Vitamins
D-Biotin	0.0002	0.002	0.0002	0.002	0.002	0.0002	0.0002
Choline Chloride	0.003	0.03	0.003	0.003	0.003	0.003	0.003
Folic Acid	0.001	—	0.001	0.001	0.001	0.001	0.001
myo-Inositol	0.035	0.35	0.035	0.035	0.035	0.035	0.035
Niacinamide	0.001	0.01	0.001	0.001	0.001	0.001	0.001
p-Aminobenzoic Acid	0.001	0.01	0.001	0.001	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.00025	0.0025	0.00025	0.00025	0.00025	0.00025	0.00025
Pyridoxine • HCl	0.001	0.01	0.001	0.001	0.001	0.001	0.001
Riboflavin	0.0002	0.002	0.0002	0.0002	0.0002	0.0002	0.0002
Thiamine • HCl	0.001	0.01	0.001	0.001	0.001	0.001	0.001
Vitamin B12	0.000005	0.00005	0.000005	0.000005	0.000005	0.000005	0.000005
Other
D-Glucose	2	20	—	2	2	2	2
Glutathione (reduced)	0.001	0.01	0.001	0.001	0.001	0.001	0.001
Phenol Red • Na	0.0053	0.053	0.0053	—	0.0053	0.0053	0.0053
Add
L-Glutamine	0.3	0.3 at 1×	—	—	—	—	—
Sodium Bicarbonate	—	2.0 at 1×	2	—	—	2	—

RPMI-1640 Medium Dutch Modification

Component	R7638 [1x] g/L
Inorganic Salts
Calcium Nitrate • 4H2O	0.1
Magnesium Sulfate (anhydrous)	0.04884
Potassium Chloride	0.4
Sodium Bicarbonate	1
Sodium Chloride	6.4
Sodium Phosphate Dibasic (anhydrous)	0.8
Amino Acids
L-Arginine	0.2
L-Asparagine (anhydrous)	0.05
L-Aspartic Acid	0.02
L-Cystine • 2HCl	0.0652
L-Glutamic Acid	0.02
Glycine	0.01
L-Histidine	0.015
Hydroxy-L-Proline	0.02
L-Isoleucine	0.05
L-Leucine	0.05
L-Lysine • HCl	0.04
L-Methionine	0.015
L-Phenylalanine	0.015
L-Proline	0.02
L-Serine	0.03
L-Threonine	0.02
L-Tryptophan	0.005
L-Tyrosine • 2Na • 2H2O	0.02883
L-Valine	0.02
Vitamins
D-Biotin	0.0002
Choline Chloride	0.003
Folic Acid	0.001
myo-Inositol	0.035
Niacinamide	0.001
p-Aminobenzoic Acid	0.001
D-Pantothenic Acid (hemicalcium)	0.00025
Pyridoxine • HCl	0.001
Riboflavin	0.0002
Thiamine • HCl	0.001
Vitamin B12	0.000005
Other
D-Glucose	2
Glutathione (reduced)	0.001
HEPES	4.77
Phenol Red • Na	0.0053
Add
L-Glutamine	0.3

RPMI-1640 Medium HEPES Modification

Component	R4130 g/L	R5886 [1x] g/L	R8005 g/L
Inorganic Salts
Calcium Nitrate • 4H2O	0.1	0.1	0.1
Magnesium Sulfate (anhydrous)	0.04884	0.04884	0.04884
Potassium Chloride	0.4	0.4	0.4
Sodium Bicarbonate	—	2	—
Sodium Chloride	6	6	5.9
Sodium Phosphate Dibasic (anhydrous)	0.8	0.8	0.8
Amino Acids
L-Arginine	0.2	0.2	0.2
L-Asparagine (anhydrous)	0.05	0.05	0.05
L-Aspartic Acid	0.02	0.02	0.02
L-Cystine • 2HCl	0.0652	0.0652	0.0652
L-Glutamic Acid	0.02	0.02	0.02
L-Glutamine	0.3	—	0.3
Glycine	0.01	0.01	0.01
L-Histidine	0.015	0.015	0.015
Hydroxy-L-Proline	0.02	0.02	0.02
L-Isoleucine	0.05	0.05	0.05
L-Leucine	0.05	0.05	0.05
L-Lysine • HCl	0.04	0.04	0.04
L-Methionine	0.015	0.015	0.015
L-Phenylalanine	0.015	0.015	0.015
L-Proline	0.02	0.02	0.02
L-Serine	0.03	0.03	0.03
L-Threonine	0.02	0.02	0.02
L-Tryptophan	0.005	0.005	0.005
L-Tyrosine • 2Na • 2H2O	0.02883	0.02883	0.02883
L-Valine	0.02	0.02	0.02
Vitamins
D-Biotin	0.0002	0.0002	0.0002
Choline Chloride	0.003	0.003	0.003
Folic Acid	0.001	0.001	0.001
myo-Inositol	0.035	0.035	0.035
Niacinamide	0.001	0.001	0.001
p-Aminobenzoic Acid	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.00025	0.00025	0.00025
Pyridoxine • HCl	0.001	0.001	0.001
Riboflavin	0.0002	0.0002	0.0002
Thiamine • HCl	0.001	0.001	0.001
Vitamin B12	0.000005	0.000005	0.000005
Other
D-Glucose	2	2	4.5
Glutathione (reduced)	0.001	0.001	0.001
HEPES	5.96	5.96	3.5745
Phenol Red • Na	0.0053	0.0053	0.0053
Add
L-Glutamine	—	0.3	—
Sodium Bicarbonate	2	—	2

RPMI-1640 Medium Modified

Component	R7388 [1x] g/L	R7509 [1x] g/L	R7513 [1x] g/L	R8755 g/L
Inorganic Salts
Calcium Nitrate • 4H2O	0.1	0.1	0.1	0.1
Magnesium Sulfate (anhydrous)	0.04884	0.04884	0.04884	0.04884
Potassium Chloride	0.4	0.4	0.4	0.4
Sodium Bicarbonate	—	2	2	—
Sodium Chloride	6	6	6	6
Sodium Phosphate Dibasic (anhydrous)	0.8	0.8	0.8	0.8
Amino Acids
L-Arginine	0.2	0.2	0.2	0.2
L-Asparagine (anhydrous)	0.05	0.05	0.05	0.05
L-Aspartic Acid	0.02	0.02	0.02	0.02
L-Cystine • 2HCl	0.0652	0.0652	—	0.0652
L-Glutamic Acid	0.02	0.02	0.02	0.02
L-Glutamine	0.3	—	—	0.3
Glycine	0.01	0.01	0.01	0.01
L-Histidine	0.015	0.015	0.015	0.015
Hydroxy-L-Proline	0.02	0.02	0.02	0.02
L-Isoleucine	0.05	0.05	0.05	0.05
L-Leucine	0.05	0.05	0.05	0.05
L-Lysine • HCl	0.04	0.04	0.04	0.04
L-Methionine	0.015	0.015	—	0.015
L-Phenylalanine	0.015	0.015	0.015	0.015
L-Proline	0.02	0.02	0.02	0.02
L-Serine	0.03	0.03	0.03	0.03
L-Threonine	0.02	0.02	0.02	0.02
L-Tryptophan	0.005	0.005	0.005	0.005
L-Tyrosine • 2Na • 2H2O	0.02883	0.02883	0.02883	0.02883
L-Valine	0.02	0.02	0.02	0.02
Vitamins
D-Biotin	0.0002	0.0002	0.0002	0.0002
Choline Chloride	0.003	0.003	0.003	0.003
Folic Acid	0.001	0.001	0.001	0.001
myo-Inositol	0.035	0.035	0.035	0.035
Niacinamide	0.001	0.001	0.001	0.001
p-Aminobenzoic Acid	0.001	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.00025	0.00025	0.00025	0.00025
Pyridoxine • HCl	0.001	0.001	0.001	0.001
Riboflavin	0.0002	0.0002	0.0002	0.0002
Thiamine • HCl	0.001	0.001	0.001	0.001
Vitamin B12	0.000005	0.000005	0.000005	0.000005
Other
D-Glucose	2	2	2	2
Glutathione (reduced)	0.001	0.001	0.001	0.001
HEPES	4.77	—	—	—
Phenol Red • Na	0.0053	—	0.0053	—
Add
L-Cystine • 2HCl	—	—	0.0652	—
L-Glutamine	—	0.3	0.3	—
L-Methionine	—	—	0.015	—
Sodium Bicarbonate	2	—	—	2

RPMI-1640 Medium Auto-Mod™ Modified for Autoclaving

Component	R7755 g/L
Inorganic Salts
Calcium Nitrate • 4H2O	0.1
Magnesium Sulfate (anhydrous)	0.04884
Potassium Chloride	0.4
Sodium Chloride	6
Sodium Phosphate Dibasic (anhydrous)	0.8
Succinic Acid • 6H2O • Na	0.1
Succinic Acid (free acid)	0.075
Amino Acids
L-Arginine	0.2
L-Asparagine (anhydrous)	0.05
L-Aspartic Acid	0.02
L-Cystine • 2HCl	0.0652
L-Glutamic Acid	0.02
Glycine	0.01
L-Histidine	0.015
Hydroxy-L-Proline	0.02
L-Isoleucine	0.05
L-Leucine	0.05
L-Lysine • HCl	0.04
L-Methionine	0.015
L-Phenylalanine	0.015
L-Proline	0.02
L-Serine	0.03
L-Threonine	0.02
L-Tryptophan	0.005
L-Tyrosine	0.02
L-Valine	0.02
Vitamins
D-Biotin	0.0002
Choline Bitartrate	0.00544
Folic Acid	0.001
myo-Inositol	0.035
Niacinamide	0.001
p-Aminobenzoic Acid	0.001
D-Pantothenic Acid (hemicalcium)	0.00025
Pyridoxine • HCl	0.001
Riboflavin	0.0002
Thiamine • HCl	0.001
Vitamin B12	0.000005
Other
D-Glucose	2
Glutathione (reduced)	0.001
Phenol Red • Na	0.00318
Add
L-Glutamine	0.3
Sodium Bicarbonate	2