UHPLC/MS Analysis of Cannabinoids Extracted from Raw Cannabis Sativa (Marijuana) on Ascentis^® Express RP-Amide

Conditions

Sample Preparation	A 100 mg sample of dried cannabis sativa leaves was extracted with 50 mL of ethanol:water (50:50, v/v) for 15 mins in an ultrasonic bath. (The sample was then filtered through a 0.45 µM Millex® LH filter prior to LC/MS analysis.)
Column	Ascentis® Express RP-Amide, 10 cm x 2.1 mm I.D., 2.0 µm particles (53913-U)
Column Temp.	35 °C
Mobile phase	[A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile
Gradient	60% B to 100% B in 10 min
Flow Rate	0.4 mL/min
Pressure	7600 psi (524 bar)
Injection	1 µL
Detector	MS, ESI(+), ESI(-), MRM

Description

Analysis Note	Cannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, inflammatory diseases, and other health conditions. Shown here is a raw cannabis sample extracted using a simple procedure followed by LC/MS/MS analysis. Eight major cannabis compounds were detected. Reversed-phase HPLC separation was achieved on an Ascentis Express RP-Amide column. Dried cannabis sample was obtained courtesy of Dr. Hari H. Singh, Program Director at the Chemistry & Physiological Systems Research Branch of the National Institute on Drug Abuse at the US National Institute of Health. The extract strain of the sample was not known.
Categories	Analytical Chromatography, Pharmaceuticals, Herbal medicines, Drugs of Abuse
Featured Industry	Food and Beverages Forensics and Toxicology Vitamins, Nutraceuticals, and Natural Products
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Suitability	Application for UHPLC-MS