GC Analysis of Volatiles in Milk Thistle Honey on SUPELCOWAX 10 after Dynamic Headspace (DHS) using 20/35 Tenax^® TA Tube

sample/matrix

25 g milk thistle honey in a 200 mL Erlenmeyer flask at 45 °C, purged with nitrogen at 60 mL/min for 30 min as a stripping gas to collect onto a Tenax^® TA tube

thermal desorption tube

20/35 Tenax^® TA, 90 mg

dry purge

back-flushed with nitrogen, 60 mL/min at room temperature for 5 min

desorption process

helium, 10 mL/min at 280 °C for 10 min to cryofocus onto a glass-lined tube at -120 °C, which was subsequently heated to 240 °C for transfer to the GC column

transfer line/valve temp.

250 °C, transfer line directly coupled to the GC column using a press-fit connector

column

SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 μm (24079)

oven

35 °C (8 min), 6 °C/min to 60 °C, 4 °C/min to 160 °C, 20 °C/min to 200 °C (1 min)

MSD interface

230 °C

scan range

35-300 m/z

carrier gas

helium, 1 mL/min