Immobilization of proteins on agarose beads, monitored in real time by bead injection spectroscopy.

Immobilization of proteins on agarose beads, monitored in real time by bead injection spectroscopy.

The Analyst (2006-06-28)

Jaromir Ruzicka, Andrea D Carroll, Ilkka Lähdesmäki

PMID16802025

RÉSUMÉ

A novel approach to real-time monitoring of protein immobilization resulted in the surprising finding that current immobilization protocols are far from optimized.

MATÉRIAUX

Référence du produit

Marque

Description du produit

L6876

Sigma-Aldrich

Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein

I8640

Sigma-Aldrich

IgG from human serum, technical grade, ≥80% (SDS-PAGE), buffered aqueous solution