- Simple, rapid, and quantitative release of periplasmic proteins by chloroform.
Simple, rapid, and quantitative release of periplasmic proteins by chloroform.
Journal of bacteriology (1984-12-01)
G F Ames, C Prody, S Kustu
PMID6501229
RÉSUMÉ
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.