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  • Quantitative determination of salidroside and tyrosol from the underground part of Rhodiola rosea by high performance liquid chromatography.

Quantitative determination of salidroside and tyrosol from the underground part of Rhodiola rosea by high performance liquid chromatography.

Archives of pharmacal research (2000-09-08)
P T Linh, Y H Kim, S P Hong, J J Jian, J S Kang
RÉSUMÉ

A reversed-phase high performance liquid chromatographic method was developed to determine salidroside and tyrosol simultaneously in the Rhodiola rosea. The optimum condition was Nova-Pak C18 as stationary phase, 6.5% methanol in water as mobile phase and detection at UV 225 nm. The identification was carried out by comparing the retention time and IC/MS spectrum of the relevant peaks with those of isolated standards. The contents of salidroside and tyrosol in the samples gathered from various area in China were ranged over 1.3-11.1 mg/g and 0.3-2.2 mg/g, respectively.

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Supelco
Salidroside, analytical standard