Accéder au contenu
Merck

Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans.

Antimicrobial agents and chemotherapy (2008-09-17)
Nathan P Wiederhold, Jodi L Grabinski, Guillermo Garcia-Effron, David S Perlin, Samuel A Lee
RÉSUMÉ

Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Sérum humain, from human male AB plasma, USA origin, sterile-filtered
Sigma-Aldrich
Sérum humain, Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered
Sigma-Aldrich
Sérum humain, (from male AB clotted whole blood), USA origin, sterile-filtered
Sigma-Aldrich
Sérum humain, Heat Inactivated (from male AB clotted whole blood), USA origin, sterile-filtered
Sigma-Aldrich
Sérum humain, from platelet poor human plasma, sterile-filtered, (mycoplasma tested, virus tested)