Power Generation by Selective Self-Assembly of Biocatalysts.

Through a careful chemical and bioelectronic design we have created a system that uses self-assembly of enzyme-nanoparticle hybrids to yield bioelectrocatalytic functionality and to enable the harnessing of electrical power from biomass. Here we show that mixed populations of hybrids acting as catalyst carriers for clean energy production can be efficiently stored, self-assembled on functionalized stationary surfaces, and magnetically re-collected to make the binding sites on the surfaces available again. The methodology is based on selective interactions occurring between chemically modified surfaces and ligand-functionalized hybrids. The design of a system with minimal cross-talk between the particles, outstanding selective binding of the hybrids at the electrode surfaces, and direct anodic and cathodic electron transfer pathways leads to mediator-less bioelectrocatalytic transformations which are implemented in the construction of a fast self-assembling, membrane-less fructose/O2 biofuel cell.