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Serine (threonine) phosphatase(s) acting on cAMP-dependent phosphoproteins in mammalian mitochondria.

FEBS letters (2002-02-20)
Anna Signorile, Anna Maria Sardanelli, Rosanna Nuzzi, Sergio Papa
RÉSUMÉ

Immunochemical and functional evidence showing the existence in the inner membrane and matrix fraction of mammalian mitochondria of serine/threonine phosphatases acting on cAMP-dependent phosphoproteins is presented. Mg(2+)-dependent Ca(2+)-inhibitable PP2C phosphatase, associated to the inner membrane, dephosphorylates the 18 kDa (NDUFS4 gene) of complex I.

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Kit pour essais sur la Ser/Thr phosphatase 1 (K-R-pT-I-R-R), Ser/Thr Phosphatase Assay Kit 1used to detect PP2A activity by either dephosphorylation of the phosphopeptide.