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Key Documents

GE29-0181-82

HiPrep™ Q High Performance 16/10

Cytiva 29-0181-82, pack of 1 column

Synonyme(s) :

HiPrep Q Column, High Performance Q Column

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About This Item

Code UNSPSC :
13111000
Nomenclature NACRES :
NA.56

ligand

quaternary amine

Description

Ion Exchanger Type (value)

Conditionnement

pack of 1 column

Fabricant/nom de marque

Cytiva 29-0181-82

Paramètres

1-5 ml/min flow rate
44 psi

Taille du lit

16 mm × 100 mm

D.I. de la colonne

16 mm

Matrice

6% cross-linked agarose

Taille des particules

24-44 μm

Taille moy. des particules

34 μm

cleaning

1-14

working range

2-12

Adéquation

suitable for bioprocess medium

Description générale

HiPrep Q HP is a 20 mL strong anion exchange chromatography column for high resolution small-scale protein purification. The Q functional group is a quaternary amino group coupled to the matrix via chemically stable ether linkages. Q Sepharose High Performance is a strong anion exchanger charged with high loading capacity over broad pH ranges.

Caractéristiques et avantages

  • Packed with Q Sepharose High Performance strong quarternary ammonium anion exchange resin.
  • Small (34 µm) bead size delivers high-performance, high-resolution purifications.
  • 20 mL HiPrep column format compatible with single-pump configurations and chromatography systems such as ÄKTA systems.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiPrep is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocoles

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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