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Key Documents

G005397

HPLC Analysis of Ginsenoside Standards on Ascentis® Express C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A]: water; [B]: acetonitrile
gradient: held at 25% for 1.5 min; 25 to 85% B in 12 min; held at 85% B for 1 min
flow rate: 1.0 mL/min
pressure: 3481 psi (240 bar)
column temp.: 60 °C
detector: UV, 205 nm
injection: 25 μL
sample: 50 μg/mL in water:methanol (82:18)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of ginseng.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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