58449AST
CHIRALPAK® HSA HPLC Column
5 μm particle size, L × I.D. 5 cm × 4 mm
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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material
stainless steel column
Quality Level
Agency
suitable for USP L79
product line
CHIRALPAK®
packaging
pkg of 1 ea
manufacturer/tradename
CHIRALPAK®
parameter
137 bar pressure (2000 psi)
20-30 °C temperature
40 °C max. temp.
technique(s)
HPLC: suitable
L × I.D.
5 cm × 4 mm
matrix
fully porous particle
matrix active group
human serum albumin phase
particle size
5 μm
operating pH
2-8
separation technique
chiral
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General description
CHIRALPAK HSA, which uses human serum albumin as the chiral selector, is highly selective for acidic racemates, preferably weak and strong acids, zwitterionic and non-protolytic (neutral) compounds. Phosphate buffers (normally 0.01-0.1M, pH 5-7) with addition of organic modifiers are used as mobile phases. Enantioselectivity and retention can be regulated by changing the mobile phase composition. However, the primary use of CHIRALPAK HSA is for fast drug/protein binding studies (1). To calculate the % protein binding, measure the retention time of an unretained compound (t0) and the compound of interest (tr) on the CHIRALPAK HSA column. Then use the capacity factor equation:
k = (tr - t0)/tr
to calculate the % protein binding (P):
P = 100k/(k+1)
Different types of mobile phases can be used. A mobile phase consisting of 6% 2-propanol in 20 mM potassium phosphate buffer, pH 7.0 gives data in good agreement with literature data. The mobile phase conditions should be chosen to suit the drugs to be tested, i.e., for high protein binding drugs a mobile phase with higher eluting strength might be needed in order to reduce retention times.
(1) Goodman, A.; Gilman, A.G. The Pharmacological Basis of Therapeutics, 9th Edition, McGraw-Hill: New York, 1996; pp 1712-1792.
k = (tr - t0)/tr
to calculate the % protein binding (P):
P = 100k/(k+1)
Different types of mobile phases can be used. A mobile phase consisting of 6% 2-propanol in 20 mM potassium phosphate buffer, pH 7.0 gives data in good agreement with literature data. The mobile phase conditions should be chosen to suit the drugs to be tested, i.e., for high protein binding drugs a mobile phase with higher eluting strength might be needed in order to reduce retention times.
(1) Goodman, A.; Gilman, A.G. The Pharmacological Basis of Therapeutics, 9th Edition, McGraw-Hill: New York, 1996; pp 1712-1792.
Legal Information
CHIRALPAK is a registered trademark of Daicel Corp.
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