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SIALICQ

Sigma-Aldrich

Sialic Acid Quantitation Kit

Sufficient for 25 reactions

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About This Item

UNSPSC Code:
41116107
NACRES:
NA.32

shipped in

wet ice

storage temp.

2-8°C

General description

The Sialic Acid Quantitation Kit provides a rapid and accurate determination of total N-acetylneuraminic acid (NANA; sialic acid). NANA is released from glycoconjugates using neuraminidase. The kit uses α(2→3,6,8,9) neuraminidase to cleave all NANA linkages, including α(2→8) and α(2→9) linkages, as well as branched NANA, for the most accurate determination of total sialic acid content.

Application

Contains all reagents to quantitate the amount of N-acetylneuraminic acid, either free, or in glycoproteins, cell surface glycoproteins, polysialic acids and capsular polysaccharides (consisting only of polysialic acid).

Quantity

Sufficient reagents are provided to perform 25 reactions, including control samples. Each reaction will measure 1-200 nmoles of NANA.

Kit Components Only

Product No.
Description
  • N-Acetylneuraminic acid aldolase 25 μL
  • N-Acetylneuraminic acid 200 μL
  • α(2→3,6,8,9)-Neuraminidase 25 μL
  • Fetuin, bovine .5 mg
  • Lactic dehydrogenase 25 μL
  • β-NADH disodium salt 3 vial(s)
  • Tris-HCl, pH 7.5 1 mL
  • Sialidase buffer 500 μL
See All (8)

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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Corina Daniela Ene et al.
Metabolites, 11(1) (2020-12-31)
Posttranslational modifications are dynamic enzymatic-mediated processes, regulated in time and space, associated with cancer development. We aimed to evaluate the significance of posttranslational modifications in the pathogenesis of clear cell renal cell carcinoma. The authors developed a prospective, observational study
Analysis of glycoprotein-associated oligosaccharides.
R A Dwek et al.
Annual review of biochemistry, 62, 65-100 (1993-01-01)
Pragasam Viswanathan et al.
Urological research, 39(4), 269-282 (2011-01-14)
Tamm-Horsfall protein (THP) is thought to protect against calcium oxalate monohydrate (COM) stone formation by inhibiting COM aggregation. Several studies reported that stone formers produce THP with reduced levels of glycosylation, particularly sialic acid levels, which leads to reduced negative
A P Corfield et al.
Biochimica et biophysica acta, 744(2), 121-126 (1983-04-28)
The anomeric specificity of six sialidases (Vibrio cholerae, Arthrobacter ureafaciens, Clostridium perfringens, Newcastle disease virus, fowl plague virus and influenza A2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in

Articles

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field.

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