RAB1317

Human Cathepsin D ELISA

for serum, plasma and cell culture supernatants

• Synonym(s): Cathepsin D
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable
• input: sample type cell culture supernatant(s); sample type plasma; sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 50 pg/mL; standard curve range: 0.049-12 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CTSD(1509)

Description

General description

This ELISA antibody pair detects human Cathepsin D. Other species not yet determined.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Biochem/physiol Actions

Cathepsin D encoded by CTSD gene, is an aspartyl protease, plays a major role in degradation of intracellular and endocytosed protein, for maintenance of tissue homeostasis and metabolism. Cathepsin D works as an endopeptidase in the acidic environment of lysosomes. Cathepsin D is part of signal transduction and a potential target for various cancer. Increased levels of Cathepsin D is associated with type 2 diabetes. Cathepsin D regulates sphingolipid metabolism and cholesterol trafficking. Genetic variations in Cathepsin D gene is a risk factor for Alzheimer′s disease and neuronal ceroid lipofuscinosis. Cathepsin D is a key marker in atherosclerosis and level of expression is increased in atherosclerotic lesions. Cathepsin D regulates the degradation of advanced glycation end products (AGEs) and impaired activity of cathepsin D results in accumulation of AGEs in photoaged skin. Increased plasma concentration of Cathepsin D causes non-alcoholic steatohepatitis (NASH) leading to hepatic lipid accumulation and inflammation.

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable