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RAB0309

Sigma-Aldrich

Mouse IL-6 ELISA Kit

for cell and tissue lysates

Synonym(s):

B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-600 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Il6(24498)

General description

The Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-6 cell lysate and tissue lysate.

IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3.

Immunogen

Recombinant Mouse IL-6

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse IL-6 ELISA Kit has been used to measure the concentration of interleukin-6 (IL-6) in cell culture supernatants.

Biochem/physiol Actions

IL-6 (interleukin 6) is a pleiotropic cytokine that mediates immune and inflammatory responses. It synergizes with IL-3 in megakaryocyte development and platelet production. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease and plasmacytoma/multiple myeloma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABLYSIS12X Cell Lysis Buffer (Item J)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Common variants in genes that mediate immunity and risk of multiple myeloma.
Brown E E, et al.
International Journal of Cancer. Journal International Du Cancer, 120(12), 2715-2722 (2007)
Metabolic induction of trained immunity through the mevalonate pathway.
Bekkering S, et al.
Cell, 172(1), 135-146 (2018)
Christopher T Turner et al.
The Journal of investigative dermatology, 139(4), 930-939 (2018-11-06)
Granzyme K (GzmK), traditionally described as a pro-apoptotic, granule-secreted serine protease, has been proposed to promote inflammation. Found at low levels in the plasma of healthy individuals, GzmK is markedly elevated in response to sepsis and infection. In this study
Interleukin-6: discovery of a pleiotropic cytokine.
Kishimoto T.
Arthritis Research & Therapy, 8(2), S2-S2 (2006)
Ciba Foundation Symposium (1992)
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