P8849
Protease Inhibitor Cocktail
for use in purification of Histidine-tagged proteins, DMSO solution
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General description
DMSO solution designed for use in the purification of histidine-tagged proteins.
The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.
The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.
Specificity
Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.
Application
The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.
It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.
It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.
Features and Benefits
Specifically formulated for histidine-tagged protein purification.
Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.
Compatibility with IMAC applications due to omission of chelating agents.
Supplied in convenient packaging options.
Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.
Compatibility with IMAC applications due to omission of chelating agents.
Supplied in convenient packaging options.
Components
AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Bestatin
E-64
Pepstatin A
Phosphoramidon
Quantity
One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.
This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins
This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins
Preparation Note
This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
185.0 °F - closed cup
Flash Point(C)
85 °C - closed cup
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