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P7367

Sigma-Aldrich

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE), for proteomics

Synonym(s):

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

Pricing and availability is not currently available.

biological source

bacterial (Elizabethkingia meningoseptica)

Quality Level

conjugate

(N-linked)

grade

for proteomics

product line

BioReagent

Assay

≥95% (SDS-PAGE)

form

powder

shelf life

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)
≥1 yr at 2‑8 °C
Solution is stable for at least 3 freeze-thaw cycles

mol wt

~36 kDa

concentration

≥300 units/mL
≥50 units/mL

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This Item
G5166F8435P9120
assay

≥95% (SDS-PAGE)

assay

-

assay

-

assay

-

grade

for proteomics

grade

-

grade

Proteomics Grade

grade

-

concentration

≥300 units/mL, ≥50 units/mL

concentration

-

concentration

-

concentration

-

form

powder

form

buffered aqueous solution

form

lyophilized powder

form

-

optimum pH

~8.6

optimum pH

-

optimum pH

-

optimum pH

-

General description

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Application

PNGase F from Elizabethkingia meningoseptica has been used:
  • for de-N-glycosylation of Zika20virus E protein[1]
  • to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells[2]
  • to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)[3]

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis
Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Katherine J D Ashbourne Excoffon et al.
    Journal of virology, 81(11), 5573-5578 (2007-03-23)
    The coxsackievirus and adenovirus receptor (CAR) is both a viral receptor and homophilic adhesion protein. The extracellular portion of CAR consists of two immunoglobulin (Ig)-like domains, each with a consensus sequence for N-glycosylation. We used chemical, genetic, and biochemical studies
    Lars Andresen et al.
    Journal of immunology (Baltimore, Md. : 1950), 188(4), 1847-1855 (2012-01-10)
    NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation
    Mikael B L Winkler et al.
    Cell, 179(2), 485-497 (2019-09-24)
    Niemann-Pick type C (NPC) proteins are essential for sterol homeostasis, believed to drive sterol integration into the lysosomal membrane before redistribution to other cellular membranes. Here, using a combination of crystallography, cryo-electron microscopy, and biochemical and in vivo studies on the
    Giel Detienne et al.
    Experimental gerontology, 60, 129-135 (2014-12-03)
    Royalactin is a glycoprotein essential for the development of long-lived queen honeybees. Only larvae fed with royal jelly, containing royalactin, develop into queens. Royalactin plays a central role in this process by switching on the epidermal growth factor (EGF) receptor
    Yuzo Fujikura et al.
    BMC biochemistry, 12, 52-52 (2011-09-23)
    Hemojuvelin (HJV) is one of essential components for expression of hepcidin, a hormone which regulates iron transport. HJV is mainly expressed in muscle and liver, and processing of HJV in both tissues is similar. However, hepcidin is expressed in liver

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