P7059

Phosphate Buffered Saline

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

• Synonym(s): Phosphate buffered saline, PBS
• MDL number: MFCD00131855
• UNSPSC Code: 12161700
• eCl@ss: 32129211
• NACRES: NA.25

Properties

• product name: Phosphate Buffered Saline, 10× PBS for Western blots and IP
• grade: for molecular biology
• Quality Level: 200
• sterility: 0.2 μm filtered
• form: liquid
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• pH: 7.2-7.6(1x solution)
• storage temp.: room temp

Description

General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Components

10x PBS contains:

• 30mM sodium phosphate
• 1.5 M sodium chloride
• 10.5 mM potassium phosphate

Preparation Note

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable