P6871

Anti-PRMT1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Protein Arginine Methyl Transferase 1 (NQ-15)
• MDL number: MFCD04975120
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using paraformaldehyde-fixed 293T cells; microarray: suitable; western blot: 0.25-0.5 μg/mL using 293T cell extracts
• UniProt accession no.: Q99873
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PRMT1(3276) ; mouse ... Prmt1(15469)

Description

General description

PRMT1 belongs to the family of protein arginine methyl transferases (PRMT). These enzymes transfer the methyl group from S-adenosyl-L methionine to the guanidino nitrogen atoms of an arginine residue. PRMTs are divided in two major types, I and II. It is also known as HRMT1L2 and IR1B4. PRMT1 was isolated through its interaction with BTG1 and TIS2, proteins that are important in cell quiescence. It is a 361 amino acids protein; its splicing variants differ at the N-terminus, giving rise to three isoforms (variants 1, 2 and 3).

Immunogen

synthetic peptide corresponding to amino acids 326-341 of mouse PRMT1, conjugated to KLH via an N-terminal added cysteine residue. The sequence is conserved in human, and differs by one amino acid from the rat sequence.

Application

Anti-PRMT1 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

PRMT type 1 and 2 catalyze the formation of monomethylarginine, but differs in that Type I (including PRMT1, 3, 4, and 6) catalyzes the formation of asymmetric dimethylarginine, whereas type II (PRMT5) catalyzes the formation of symmetric dimethylarginine.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves