P2482
Monoclonal Anti-Protein Kinase Bα antibody produced in mouse
clone PKB-175, ascites fluid
Synonym(s):
Anti-Akt1, Anti-PKBα
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About This Item
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
PKB-175, monoclonal
mol wt
antigen 56 kDa
contains
15 mM sodium azide
species reactivity
chicken, human, bovine, rat, mouse
technique(s)
indirect ELISA: suitable
microarray: suitable
western blot: 1:5,000 using human MCF-7 cell extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)
General description
Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) (mouse IgG1 isotype) is derived from the PKB-175 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PKB/Akt is composed of an N-terminal pleckstrin-homology (PH) domain, followed by a catalytic kinase domain and a short C-terminal regulatory domain. This cytoplasmic protein is made up of 480 amino acids. AKT1 is mapped to human chromosome 14q32.
Protein Kinase B (also known as Akt, or RAC-PK) is a protein that belongs to serine/threonine kinases.
Specificity
Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) recognizes PKB isoform α.
Immunogen
Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.
Application
Monoclonal Anti-Protein Kinase Bα antibody produced in mouse has been used in:
- western blot
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Protein kinase Bα (PKBα/Akt1) plays a crucial role as a suppressor of apoptotic cell death in different cell types, induced by a variety of stimuli including growth factor withdrawal, loss of cell adhesion and DNA damage. It can protect cerebellar neurons from apoptosis induced by insulin-like growth factor 1 (IGF-1) withdrawal. Akt is the cellular homologue of the viral oncogene v-akt of the AKT-8 acute transforming retrovirus found in rodent T cell lymphoma. It is considered to play an important role in the control of cell cycle, cell proliferation and differentiation . AKT plays a key role in angiogenesis.
Physical form
Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) is supplied as ascites fluid containing 15 mM sodium azide
Storage and Stability
For continuous use, store at 2-8C for upto one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells
Garcia RA, et al.
Molecular Cancer, 12(1), 44-44 (2013)
Shuyi Nie et al.
Mechanisms of development, 124(9-10), 657-667 (2007-08-25)
ErbB signaling regulates cell adhesion and movements during Xenopus gastrulation, but the downstream pathways involved have not been elucidated. In this study, we show that phosphatidylinositol-3 kinase (PI3K) and Erk mitogen-activated protein kinase (MAPK) mediate ErbB signaling to regulate gastrulation.
Jinling Wu et al.
Development (Cambridge, England), 133(18), 3651-3660 (2006-08-18)
GIPC is a PDZ-domain-containing protein identified in vertebrate and invertebrate organisms through its interaction with a variety of binding partners including many membrane proteins. Despite the multiple reports identifying GIPC, its endogenous function and the physiological significance of these interactions
Rodolfo Daniel Cervantes-Villagrana et al.
The Journal of biological chemistry, 294(2), 531-546 (2018-11-18)
G protein-coupled receptors stimulate Rho guanine nucleotide exchange factors that promote mammalian cell migration. Rac and Rho GTPases exert opposing effects on cell morphology and are stimulated downstream of Gβγ and Gα12/13 or Gαq, respectively. These Gα subunits might in
P J Coffer et al.
European journal of biochemistry, 201(2), 475-481 (1991-10-15)
Highly degenerate oligonucleotide primers designed from regions conserved between protein-serine kinases have been used specifically to amplify human epithelial (HeLa) cDNA by the polymerase chain reaction (PCR). Of several novel cDNA fragments encoding putative kinases thus isolated, one was further
Articles
Quantitative and qualitative western blotting to validate knockdown by esiRNA. Sigma-Aldrich.com
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