P2014

Phosphorylase Kinase from rabbit muscle

lyophilized powder, ≥60 units/mg protein

• Synonym(s): ATP:phosphorylase-b phosphotransferase, Dephosphophosphorylase kinase
• CAS Number: 9001-88-1
• Enzyme Commission number: 2.7.1.38 (BRENDA, IUBMB)
• MDL number: MFCD00131906
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• specific activity: ≥60 units/mg protein
• composition: Protein, 20-40% biuret
• foreign activity: ATPase ≤0.5%; phosphorylase a ≤1%; phosphorylase b ≤5%
• storage temp.: −20°C

Description

General description

Phosphorylase kinase contains four subunits each containing an α, β, γ, and sigma component. The sigma component binds 4 calcium molecules and is termed calmodulin while the γ unit acts as the catalytic subunit.

Application

Phosphorylase kinase from rabbit muscle has been used in a study to assess features of glycogen phosphorylase. It has also been used in a study to investigate the activation of different forms of muscle phosphorylase kinase by actin.

Unit Definition

One unit will form 1.0 μmolar unit of phosphorylase a from phosphorylase b per min at pH 7.7 at 30°C in the presence of ATP.

Physical form

Lyophilized powder containing (NH₄)₂SO₄, sucrose, β-glycerophosphate and dithioerythritol

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves