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MAK316

Sigma-Aldrich

Heme Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Heme Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

hematological disorder

storage temp.

2-8°C

General description

Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of heme and evaluation of drug effects on heme metabolism in a variety of biological samples such as blood, plasma, serum, urine, and heme carrying enzymes.

Principle

Heme assay is based on an aqueous alkaline solution method, in which the heme is converted into a uniform colored form, producing a colorimetric (400 nm) result, directly proportional to the heme concentration in the sample. The optimized formulation reduces interference and exhibits high sensitivity. One mg/dL heme equals 15.3M, 0.001%, or 10ppm. The kit has a linear detection range of 0.6–125M heme in a 96 well format.

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Warning

Hazard Statements

H290,H319,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XIV (Authorisation List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hemopexin therapy reverts heme-induced proinflammatory phenotypic switching of macrophages in a mouse model of sickle cell disease.
Vinchi F, et al.
Blood, 127(4), 473-486 (2016)
Role of heme and heme-proteins in trypanosomatid essential metabolic pathways.
Tripodi K E, et al.
Enzyme Research, 2011 (2011)
Alfonso Rubio-Navarro et al.
Frontiers in pharmacology, 10, 740-740 (2019-07-25)
Massive intravascular hemolysis is associated with acute kidney injury (AKI). Nuclear factor erythroid-2-related factor 2 (Nrf2) plays a central role in the defense against oxidative stress by activating the expression of antioxidant proteins. We investigated the role of Nrf2 in
George T Mukosera et al.
Nitric oxide : biology and chemistry, 79, 57-67 (2018-07-31)
Dinitrosyl iron complexes (DNICs) are important intermediates in the metabolism of nitric oxide (NO). They have been considered to be NO storage adducts able to release NO, scavengers of excess NO during inflammatory hypotensive shock, and mediators of apoptosis in
Beatriz Alvarez-Castelao et al.
eLife, 9 (2020-04-25)
We examined the feedback between the major protein degradation pathway, the ubiquitin-proteasome system (UPS), and protein synthesis in rat and mouse neurons. When protein degradation was inhibited, we observed a coordinate dramatic reduction in nascent protein synthesis in neuronal cell
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