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M0650

Sigma-Aldrich

Medium 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Synonym(s):

Cell growth medium, M199 Medium, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

  • to culture human mesothelial cells
  • as a component of the holding medium for the collection of embryos from pluriparous Boer goat
  • in the preparation of spheroid-embedded collagen matrices

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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