L5135
L-Leucine Dehydrogenase from Bacillus cereus
lyophilized powder, ≥60 units/mg protein
Synonym(s):
L-Leucine:NAD+ oxidoreductase (deaminating)
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
form
lyophilized powder
Quality Level
specific activity
≥60 units/mg protein
mol wt
245 kDa
storage temp.
−20°C
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General description
L-Leucine Dehydrogenase is a member of the amino acid dehydrogenase family.
Application
L-Leucine Dehydrogenase from Bacillus cereus has been used to determine the branched-chain amino acids (BCAA) spectrophotometrically in serum samples.
Biochem/physiol Actions
Leucine Dehydrogenase is a nicotinamide adenine dinucleotide hydrogen (NADH)-dependent oxidoreductase. It is involved in catalyzing the reductive amination of aliphatic 2-oxo-acids to their respective L-amino acids.
Unit Definition
One unit will convert 1.0 μmole of L‑leucine to α-ketoisocaproate per min at pH 10.5 at 37 °C.
Other Notes
contains lysine
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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