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Merck

I5271

Sigma-Aldrich

IL-1β mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IL-1β, Lymphocyte activating factor

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

Pricing and availability is not currently available.

product name

Interleukin-1β from mouse, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

mouse

Quality Level

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤0.010 ng/mL ED50

quality

endotoxin tested

mol wt

17.5 kDa (containing 153 amino acid residues)

packaging

pkg of 5X5 μg
pkg of 5 μg

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This Item
I0523I5396I2393
assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥98% (SDS-PAGE and HPLC)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rat

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.[1]

Application

Interleukin-1β from mouse has been used:
  • as a proinflammatory agent,[2] to induce inflammatory signaling cascades mediated cellular damage[3]
  • to study its effect on fetuin A-induced inflammatory response in podocyte[4]
  • to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes[5]

Biochem/physiol Actions

Interleukin-1β (IL-1β) is a potent inflammatory cytokine,[1] and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide.[6] IL-1β release is stimulated by injury and increased levels are observed in response to severe pain.[7] It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis.[1] IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.[8]

Physical form

Lyophilized from 0.2 μm filtered solution in 10mM Sodium Citrate, pH 4.0

Analysis Note

The biological activity is measured by the dose-dependent stimulation of murine D10G4.1 cells.

Other Notes

The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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TNFalpha stimulates Map4k4 expression through TNFalpha receptor 1 signaling to cJUN and ATF2
Tesz GJ, et al.
The Journal of biological chemistry (2007)
Fetuin-A aggravates lipotoxicity in podocytes via interleukin-1 signaling
Orellana JM, et al.
Physiological Reports, 5(10), e13287-e13287 (2017)
Wuttichai Jaidee et al.
Journal of natural products, 82(2), 283-292 (2019-01-30)
Four new chalcones (1, 10, 13, and 14), a new flavanone, (9), a new amide (8), and 19 known compounds were acquired from Melodorum siamensis. The structures were established by NMR and MS data analyses. Compounds 1 (er 1.4:1) and
TNFalpha stimulates Map4k4 expression through TNFalpha receptor 1 signaling to cJUN and ATF2
Tesz GJ, et al.
Test (2007)
TNFalpha stimulates Map4k4 expression through TNFalpha receptor 1 signaling to cJUN and ATF2
Tesz GJ, et al.
The Journal of Biological Chemistry (2007)

Articles

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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