GE28-4055-00

GST MultiTrap^™ 4B

Cytiva 28-4055-00

• UNSPSC Code: 12161501
• NACRES: NA.32

Properties

• ligand: glutathione
• material: polypropylene and polyethylene
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• manufacturer/tradename: Cytiva 28-4055-00
• packaging: pkg of 4 x 96-well
• size: 127.8 mm × 85.5 mm × 30.6 mm
• matrix: 4% agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 4-13
• working range: 4-13
• capacity: >25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)

Description

General description

GST MultiTrap^™ FF and GST MultiTrap^™ 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

Application

GST MultiTrap^™ FF and GST MultiTrap^™ 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap^™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap^™ FF is prepacked with Glutathione Sepharose^™ 4 Fast Flow, while GST MultiTrap^™ 4B is prepacked with Glutathione Sepharose^™ 4B.

Features and Benefits

• 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
• Highly reproducible well-to well and plate-to-plate results.
• High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
• Wells are prepacked with Glutathione Sepharose^™ media for convenience.
• Easy and predictable scale-up using prepacked GSTrap^™ FF, GSTPrep^™ FF 16/10, and GSTrap^™ 4B columns.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GSTPrep is a trademark of Cytiva

GSTrap is a trademark of Cytiva

MultiTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids