GE17-5110-01

HiTrap^® IGM purification

Cytiva 17-5110-01, pack of 5 × 1 mL

• Synonym(s): IGM Purification Column
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: 2-mercaptopyridine
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 17-5110-01
• storage condition: (20% Ethanol)
• parameter: <4 mL/min flow rate; 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 16 mm
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-13
• working range: 3-11
• storage temp.: 2-8°C

Description

General description

HiTrap^® IgM Purification HP is a 1 mL column prepacked with Sepharose^™ High Performancefor fast and efficient purification of monoclonal IgM.

Application

HiTrap^® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Features and Benefits

• Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
• Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^™ High Performance.
• Recovery of IgM is high with excellent activity retention.
• The top and bottom frits are manufactured from porous polyethylene
• Columns are delivered with a stopper on the inlet and a snap-off end on the outle

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids