GE17-0981-01
CNBr Activated Sepharose™ 4 Fast Flow
Cytiva 17-0981-01, pack of 10 g
About This Item
Recommended Products
ligand
cyanogen bromide
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 10 g
manufacturer/tradename
Cytiva 17-0981-01
storage condition
(Unsused: 2 - 8°C - Used: 2 - 8°C in 20% Ethanol)
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-11(Ligand dependent)
working range
3-11(ligand dependent)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
General description
CNBr-activated Sepharose™ 4 Fast Flow is based on the well established Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. CNBr-activated Speharose 4 Fast Flow is designed for multipoint attachment of protein ligands containing amino groups.
The preparation and use of affinity chromatography media by coupling biospecific ligands to CNBr-activated Sepharose™ 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure.
CNBr-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, CNBr-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- CNBr-activated BioProcess medium designed for coupling of large amino-containing ligands.
- Easy, rapid and efficient coupling
- This resin allows for multi-point attachment of protein ligands which minimizes ligand leakage
- BioProcess medium supported for industrial applications and well-established in approved processes
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Certificates of Analysis (COA)
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Protocols
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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