GE17-0980-01
Butyl Sepharose™ 4 Fast Flow
Cytiva 17-0980-01, pack of 200 mL
Synonym(s):
Butyl Sepharose, Fast Flow Sepharose
About This Item
Recommended Products
ligand
butyl
packaging
pack of 200 mL
manufacturer/tradename
Cytiva 17-0980-01
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
3-13
suitability
suitable for bioprocess medium
Related Categories
General description
Application
Butyl Sepharose™ 4 Fast FLow is based on cross-linked 4% Agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
Butyl Sepharose™ 4 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Standard aliphatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
- Optimized for the separation of larger Proteins in capture and intermediate purification steps
- BioProcess medium supported for industrial applications and well-established in approved processes
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered
This page discusses column packing and preparation techniques for reverse phase chromatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature
Protocols
While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.
This page discusses various aspects of sample preparation for chromatographic purification.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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