GE17-0300-10
Ficoll® PM 400
Cytiva 17-0300-10, pack of 100 g
Synonym(s):
Density gradient medium
About This Item
Recommended Products
form
solid
spray-dried
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0300-10
color
White
solubility
soluble
General description
Features and Benefits
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
- Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
- Better osmotic properties than sucrose.
- Preserves functional and morphological integrity.
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
- Does not penetrate biological membranes.
- Serves as the raw material for preparation of Ficoll-Paque gradients.
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Certificates of Analysis (COA)
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Articles
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.
This page shows methods to remove Percoll from a sample after centrifugation.
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
Protocols
How to use percoll to prepare a gradient
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
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