Recommended Products
biological source
human embryo
growth mode
Adherent
karyotype
Diploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human embryo fibroblast
Cell Line Description
Human embryo fibroblast cell line.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9
TPOX: 9,11
vWA: 15
CSF1PO: 10,12
D13S317: 10,12
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9
TPOX: 9,11
vWA: 15
Culture Medium
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should also grow in DMEM or RPMI 1640. This cell line also grow well in HAT.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service