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Key Documents

B7405

Sigma-Aldrich

Anti-Digoxin−Biotin antibody, Mouse monoclonal

clone DI-22, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin−Biotin antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DI-22, monoclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule
western blot: 1:500 using using digoxin-labeled biomolecule

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in:
  • in situ hybridization
  • immunohistochemistry
  • immunolocalization

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.

Biochem/physiol Actions

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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