Skip to Content
Merck
All Photos(3)

Key Documents

B1155

Sigma-Aldrich

bisBenzimide H 33258

powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Synonym(s):

2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride, BBIH, BXI-72, HOE 33258, Hoechst 33258, NSC334072

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C25H24N6O · 3HCl
CAS Number:
Molecular Weight:
533.88
Beilstein:
4088183
EC Number:
MDL number:
UNSPSC Code:
41106305
PubChem Substance ID:
NACRES:
NA.77

Pricing and availability is not currently available.

product line

BioReagent

Quality Level

Assay

≥98% (HPLC and TLC)

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 10 mg/mL
water and ethanol: 10 mg/mL

suitability

passes application test for fluorescence

storage temp.

−20°C

SMILES string

Cl[H].Cl[H].Cl[H].[H]O[H].CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
EC700EC800EC400
storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

purified by

(Single-spin negative chromotography)

purified by

(Single-spin negative chromotography), (Time: 3 minutes or less)

purified by

(Single-spin negative chromotography), (Time: 3 minutes or less)

purified by

(Single-spin negative chromotography), (Time: 30 minutes or less)

feature

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)

feature

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the removal of organic solvent traces from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.)

feature

Compatible Application (Suitable for most common downstream applications, including RT-PCR, gene expression, and NGS), Intended use (For depletion of impurities from RNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.)

feature

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For purification of genomic DNA from cell lines), Typical/expected yield (Varies by sample. Please reference user guide for more information.)

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

Application

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Maria Elena Dell'Aquila et al.
    Fertility and sterility, 91(1), 249-255 (2008-03-28)
    To analyze the effects of GnRH agonists versus antagonists on mitochondrial distribution and activity in human mature oocytes. Randomized research experimental study. Academic basic research laboratory and hospital-based fertility center. Two hundred twenty-five supernumerary mature oocytes from 44 patients. Fluorescent
    Luisa Valentini et al.
    Animal reproduction science, 117(1-2), 166-177 (2009-04-18)
    This study investigates the mitochondrial (mt) distribution in canine ovarian oocytes examined at recovery time, as related to the reproductive cycle stage, and in oviductal oocytes. Ovarian Germinal Vesicle (GV) stage oocytes were recovered from bitches in anestrous (A, n=2)
    Luke O'Shaughnessy et al.
    Methods in molecular biology (Clifton, N.J.), 681, 295-309 (2010-10-28)
    Expression of recombinant proteins in the baculovirus/insect cell expression system is employed because it enables post-translational protein modification and high yields of recombinant protein. The system is capable of facilitating the functional expression of many proteins - either secreted or
    Jared J Luxton et al.
    Cell reports, 33(10), 108457-108457 (2020-11-27)
    Telomere length dynamics and DNA damage responses were assessed before, during, and after one-year or shorter duration missions aboard the International Space Station (ISS) in a comparatively large cohort of astronauts (n = 11). Although generally healthy individuals, astronauts tended to
    Luis Emiliano Peña-Altamira et al.
    Neurochemistry international, 115, 37-49 (2017-10-25)
    Purine nucleoside phosphorylase (PNP), a crucial enzyme in purine metabolism which converts ribonucleosides into purine bases, has mainly been found inside glial cells. Since we recently demonstrated that PNP is released from rat C6 glioma cells, we then wondered whether

    Articles

    Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service