A6106
Aprotinin
BioUltra, 3-8 TIU/mg solid, powder, ≥98% (SDS-PAGE), suitable for inhibition assay
Synonym(s):
BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)
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All Photos(4)
About This Item
Empirical Formula (Hill Notation):
C284H432N84O79S7
CAS Number:
Molecular Weight:
6511.44
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.77
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product name
Aprotinin from bovine lung, BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)
biological source
bovine lung
Quality Level
product line
BioUltra
Assay
≥98% (SDS-PAGE)
form
powder
specific activity
3-8 TIU/mg solid
mol wt
~6,500
technique(s)
inhibition assay: suitable
solubility
H2O: 5 mg/mL, clear to very slightly hazy
UniProt accession no.
storage temp.
2-8°C
InChI key
ZPNFWUPYTFPOJU-UHFFFAOYSA-N
Gene Information
cow ... PTI(404172)
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Application
Aprotinin is largely used as an inhibitor of trypsin.
Biochem/physiol Actions
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Unit Definition
One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.
Preparation Note
A further purification of A1153 to yield a product with slightly higher activity and purity
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
Enzymatic Assay of Aprotinin
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