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Key Documents

A0802

Sigma-Aldrich

Adipic acid dihydrazide–Agarose

saline suspension

Synonym(s):

Adipic acid dihydrazide agarose beads

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About This Item

MDL number:
MFCD00161954
UNSPSC Code:
23151817
NACRES:
NA.56

biological source

plant

form

saline suspension

extent of labeling

≥8 μmol per mL

technique(s)

microbe id | metabolite detection: suitable

matrix activation

cyanogen bromide

matrix spacer

11 atoms (when ligands (aldehydes, carboxylic acids) are coupled through free hydrazide groups)

suitability

suitable for chromatography

storage temp.

2-8°C

Application

Adipic acid dihydrazide Agarose can be used in proteomics and protein chromatography. It has been utilized in research for purifying and identifying the fungal phytotoxin Fusicoccin (FC), identifying RNA binding proteins that interact with RNA cis-elements, and enzyme purification such as peroxisomes from guinea pig liver.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A H de Boer et al.
Plant physiology, 89, 250-259 (1989-01-01)
Fusicoccin (FC), a fungal phytotoxin, stimulates the H(+) -ATPase located in the plasma membrane (PM) of higher plants. The first event in the reaction chain leading to enhanced H(+) -efflux seems to be the binding of FC to a FC-binding
D Grate et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(11), 6131-6136 (1999-05-26)
The biological function of specific gene products often is determined experimentally by blocking their expression in an organism and observing the resulting phenotype. Chromophore-assisted laser inactivation using malachite green (MG)-tagged antibodies makes it possible to inactivate target proteins in a
Ruben Hovhannisyan et al.
BioTechniques, 46(2), 95-98 (2009-03-26)
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation. These purifications can be complicated by residual RNase activity in cellular extracts that can degrade
Alice Tianbu Zhang et al.
Journal of cell science, 124(Pt 12), 2058-2069 (2011-05-26)
Non-coding Y RNAs are required for the initiation of chromosomal DNA replication in mammalian cells. It is unknown how they perform this function or if they associate with a nuclear structure during DNA replication. Here, we investigate the association of
S C Datta et al.
The Journal of biological chemistry, 265(14), 8268-8274 (1990-05-15)
The peroxisomal acyl/alkyl dihydroxyacetone-phosphate reductase (EC 1.1.1.101) was solubilized and purified 5500-fold from guinea pig liver. The enzyme could be solubilized by detergents only at high ionic strengths in presence of the cosubstrate NADPH. Peroxisomes, isolated from liver by a
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