90A1
Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit
Select reagents packaged in gelatin capsules.
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About This Item
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Quality Level
form
liquid
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
application(s)
hematology
histology
shipped in
wet ice
storage temp.
2-8°C
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Application
Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.
Kit Components Only
Product No.
Description
- α-Naphthyl acetate (90-6) 10 x 20
- Citrate solution (386-1) 20 mL
- Ethylene glycol monomethyl ether 25 mL
- Fast Blue RR Salt (FBS-25) 10 caps
- Mayer's Hematoxylin Solution (MHS-1) 100 mL
- TRIZMAL™ 7.6 Concentrate 50 mL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - STOT RE 2 - STOT SE 1
Target Organs
Immune system, thymus
Storage Class Code
3 - Flammable liquids
Flash Point(F)
104.0 °F - closed cup
Flash Point(C)
40 °C - closed cup
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