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Millipore

LST-MUG Broth

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 2.75 g/L
lactose, 5 g/L
4-methylumbelliferyl-β-D-glucuronide, 0.1 g/L
potassium dihydrogen phosphate, 2.75 g/L
sodium chloride, 5 g/L
sodium lauryl sulfate, 0.1 g/L
L-tryptophan, 1 g/L
tryptose, 20 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Escherichia coli
selective and differential for coliforms

Application

Fluorescent method for the detection of E. coli.
LST-MUG broth is a growth media, which is used for the selective detection and differentiation of Escherichia coli in water samples.

Preparation Note

Dissolve 36.5 g in 1 litre distilled water and fill into tubes which can be optionally fitted out with Durham tubes. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Fluorogenic assay for rapid detection of E. coli in chilled and frozen foods.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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L J Moberg et al.
Journal - Association of Official Analytical Chemists, 71(3), 589-602 (1988-05-01)
A collaborative study was conducted to compare a proposed LST-MUG method with the AOAC official method for Escherichia coli detection. E. coli produces an enzyme, beta-glucuronidase, which cleaves the substrate, 4-methyl-umbelliferyl-beta-D-glucuronide (MUG), to yield a fluorescent end product. Incorporation of

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