198099

Methyl violet 2B

certified by the Biological Stain Commission

• Synonym(s): Basic Violet 1
• CAS Number: 8004-87-3
• Colour Index Number: 42535
• Beilstein: 8464808
• MDL number: MFCD00012568
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851861
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 100
• form: crystalline powder
• composition: Dye content, 75%
• technique(s): microbe id | staining: suitable
• mp: 137 °C (dec.) (lit.)
• λmax: 584 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl.C\N=C1/C=C\C(C=C1)=C(/c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C
• InChI: 1S/C24H27N3.ClH/c1-25-21-12-6-18(7-13-21)24(19-8-14-22(15-9-19)26(2)3)20-10-16-23(17-11-20)27(4)5;/h6-17H,1-5H3;1H
• InChI key: JFTBTTPUYRGXDG-UHFFFAOYSA-N

Description

General description

Methyl Violet 2B is a mixture of polymethylated (4,5 and 6) pararosaniline chlorides

Application

Methyl violet 2B has been used to stain human lung fibroblast cells (A549) in in vitro antiviral assay.

Biochem/physiol Actions

Methyl violet 2B is a cationic dye with a triphenylmethane structure. It acts as a selective dye and facilitates the differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms. Methyl violet 2B is harmful if inhaled, swallowed or absorbed through skin, contact may cause damage to skin and eyes. Hence, the dye removal is of great importance.

Suitability

Certified for use in Flemming triple stain with iodine for chromosomes; Newton′s crystal violet-iodine technique for chromatin and nucleoli; Lieb′s amyloid stain.

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H318,H351,H410
• Precautionary Statements: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves