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antibody form
purified antibody
clone
MAb GEa (5-16 H1C8)
monoclonal
purified by
(Ligatrap)
species reactivity
mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgM
UniProt accession no.
General description
Purified actin (ACT1) from Arabidopsis thaliana plant.
Specificity
Clone 5-16 H1C8 is a mouse monoclonal antibody that detects Actin in a wide range of species.
Application
ELISA Analysis: A representative lot detected Actin in ELISA applications (Kandasamy, M.K., et. al. (1999). Plant J. 18(6):681-91).
Western Blotting Analysis: A representative lot detected Actin in Western Blotting applications (Kandasamy, M.K., et. al. (2012). Plant Cell. 24(5):2041-57; Kandasamy, M.K., et. al. (1999). Plant J. 18(6):681-91).
Western Blotting Analysis: A representative lot detected Actin in Western Blotting applications (Kandasamy, M.K., et. al. (2012). Plant Cell. 24(5):2041-57; Kandasamy, M.K., et. al. (1999). Plant J. 18(6):681-91).
Quality
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Actin in human skeletal muscle tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Actin in human skeletal muscle tissue lysate.
Physical properties
~44 kDa observed; 41.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgM in PBS without azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Other Notes
Human. Predicted to react with all eukaryotes based on 100% sequence homology.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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