MABS2239

Anti-CXCL9 Antibody

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: hamster
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2A6.9.9, monoclonal
• mol wt: calculated mol wt 14.44 kDa; observed mol wt ~14 kDa
• species reactivity: mouse
• packaging: antibody small pack of 100 μL
• technique(s): neutralization: suitable; western blot: suitable
• isotype: IgGκ
• UniProt accession no.: P18340
• shipped in: dry ice
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Cxcl9(17329)

Description

General description

C-X-C motif chemokine 9 (UniProt: P18340; also known as Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, MIG, MuMIG, Protein m119, Small-inducible cytokine B9) is encoded by the Cxcl9 (also known as Scyb9) gene (Gene ID: 17329) in murine species. CXCL9 is a small cytokine of the CXC chemokine family that lacks the Glu-Leu-Arg (ELR) domain. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form. It is induced in macrophages in response to interferon- g and participates in immune and inflammatory response. Its actions are mediated via CXC-chemokine receptor 3 (CXCR3). CXCL10 and CXCL11 that are structurally related to CXCL9 also serve as ligands for CXCR3 and contribute to its activation in CXCR3 expressing cells. Overexpression of CXCL9 is reported to reduced tumor progression and metastasis via the inhibition of angiogenesis. Conversely, CXCL9 can also act directly on tumor cells expressing the CXCR3 receptor to promote cell migration and epithelial mesenchymal transition.

Specificity

Clone 2A6 is an Armenian Hamster monoclonal antibody that detects murine CXCL9/MIG.

Immunogen

Recombinant mouse C-X-C motif chemokine 9 (CXCL9).

Application

Quality Control Testing

Evaluated by Western Blotting with murine recombinant CXCL9/MIG protein.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine CXCL9/MIG protein.

Tested Applications

Neutralizing Analysis: A representative lot detected CXCL9 in Neutralizing applications (Christen, U., et. al. (2003). J Immunol. 171(12):6838-45; Tajfirouz, D., et. al. (2017). Virology. 506:7-13).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CXCL9, clone 2A6.9.9, Cat. No. MABS2239, is an Armenian hamster monoclonal antibody that detects C-X-C motif chemokine 9 (CXCL9) and is tested for use in Neutralizing and Western Blotting.

Physical form

Purified Armenian hamster monoclonal antibody IgG in PBS without preservatives.

Storage and Stability

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable