MABF191
Anti-CSF1 Antibody, clone 2D10
ascites fluid, clone 2D10, from mouse
Synonym(s):
Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Processed macrophage colony-stimulating factor 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2D10, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CSF1(1435)
General description
Macrophage colony stimulating factor 1 (CSF1,CSF-1, M-CSF, MCSF) or Lanimostim is a cytokine that was first identified as a molecule that plays an important role in the survival, proliferation and differentiation of mononuclear cells such as phagocytes, macrophages and monocytes. Today however we know that CSF1 also serves several other functions in a variety of cell types. CSF1 is essential for normal bone development, osteoclast proliferation, and the resorption of bone. CSF1 is released by osteoclasts upon stimulation by parathyroid hormone. Additionally CSF1 impacts actin and cytoskeleton reorganization which effects cell adhesion and migration, and overstimulation of CSF1 in endothelial cells is linked to the development and progression of atherosclerosis. CSF1 is also highly expressed in the endometrial epithelium of the pregnant uterus and CSF1 is essential for normal embryonic implantation and placental development. CSF1 appears to play an important role in cancer as well; first CSF1 is an anti-tumor agent and increases macrophage expression of anti-tumor activity, but over expression of CSF1 in breast, bone, and several other forms of cancer appears to promote cell invasiveness, proliferation, and tumor survival. Finally, CSF1 as a secreted mature factor promotes the release of proinflammatory cytokines and serves as part of the innate immunity process for inflammation and infection. CSF1 is initially produced as a precursor that is membrane bound but processed and secreted upon stimulation. CSF1 is heavily glycosylated and found both in the ECM as well as surface membranes of cells. CSF1 receptor is found on numerous cell types including macrophages, endothelial cells, and osteoclasts. EMD-Millipore’s Anti-CSF1 mouse monoclonal antibody has been tested in western blot on recombinant protein and in paraffin embedded immunohistochemistry on human placental tissue sections.
Immunogen
Purified recombinant fragment of human CSF1 expressed in E. Coli.
Application
Anti-CSF1 Antibody, clone 2D10 is a highly specific mouse monoclonal antibody, that targets CSF1 & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CSF1 in human placenta tissue.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunological Signaling
Immunological Signaling
Quality
Evaluated by Western Blotting in human recombinant CSF1 protein.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected human recombinant CSF1 protein.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected human recombinant CSF1 protein.
Target description
~60 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Human recombinant CSF1 protein
Human recombinant CSF1 protein
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Wei-Chi Hsu et al.
Disease markers, 2019, 2724948-2724948 (2019-10-01)
Colony-stimulating factor-1 (CSF-1) is a homodimeric glycoprotein. The main role of CSF-1 is as a hematopoietic growth factor that modulates proliferation, differentiation, and survival of macrophages. Moreover, CSF-1 has also been reported to be aberrantly expressed in several human cancers.
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