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MAB5386X

Sigma-Aldrich

Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor™ 488

clone ME20.4, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ME20.4, monoclonal

species reactivity

rabbit, human, pig, sheep

should not react with

mouse, chicken, rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NM_002507.2

UniProt accession no.

P08138

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NGFR(4804)

General description

The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells

Specificity

Reacts with the low affinity nerve growth factor receptor (NGFR p75). The epitope recognized by the antibody maps within the third or fourth cysteine-rich repeats of the NGFR molecule. Reactivity has not been observed with rat, chicken, or mouse NGFR.

Immunogen

Human melanoma cell line.

Application

This Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor 488 is validated for use in FC, IC for the detection of Nerve Growth Factor Receptor.

Storage and Stability

Aliquot and store at -20 ºC, or glycerol (ASC grade or better) can be added (1:1) and the antibody stored at -20ºC for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L A Tremere et al.
Neuroreport, 11(10), 2177-2183 (2000-08-03)
192-IgG is an antibody directed against the p75 low affinity nerve growth factor receptor in rats, whereas ME 20.4 was raised against the analogous protein in humans. Coupled to saporin, 192-IgG and ME 20.4 have been used to lesion basal
G Ferreira et al.
Neuroscience, 104(2), 419-439 (2001-05-30)
The basal forebrain cholinergic system is involved in different forms of memory. To study its role in social memory in sheep, an immunotoxin, ME20.4 immunoglobulin G (IgG)-saporin, was developed that is specific to basal forebrain cholinergic neurons bearing the p75

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