GF314
TNF- α Protein, Human Recombinant Animal Free
Tumor necrosis factor alpha (TNF-α), also known as cachectin & TNFSF1A, is the prototypic ligand of the TNF superfamily. Recombinant animal free human TNF-α is manufactured using all non-animal reagents.
Synonym(s):
Tumor Necrosis Factor Alpha, TNFa, TNF-alpha, Cachectin, TNFSF1A
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
>98% (SDS-PAGE and HPLC)
impurities
<0.1 μg/mg protein endotoxin (<1EU/μg)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Related Categories
General description
Product Source: E. coli
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Specificity
Cross Reactivty
Cow, Monkey, Mouse, Rat, Sheep
Cow, Monkey, Mouse, Rat, Sheep
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Quality
Bioactivity assay: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥2 x 107 units/mg.
Physical form
Product is filtered through a 0.2 micron filter before lyophilization.
Recombinant animal free human TNF- α is manufactured using all non-animal reagents
Storage and Stability
Store at -20°C for up to 6 months from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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Cell death & disease, 13(7), 633-633 (2022-07-22)
Uncontrolled growth, distant metastasis and chemoresistance are critical characteristics of pancreatic ductal adenocarcinoma (PDAC), and they result in high mortality; however, the mechanisms triggering these effects have not been fully investigated. In this study, we analysed a dataset in the
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